相关实验视频
Updated: Apr 11, 2026
10:28
Author Spotlight: RNAi Inheritance and ChIP in C. elegans
Published on: May 5, 2023
5.0K
这是基因沉默. 由HUSH复合体进行的表观遗传沉默会调解人体细胞中的位置效应变异
Iva A Tchasovnikarova1, Richard T Timms1, Nicholas J Matheson1
1Department of Medicine, Cambridge Institute for Medical Research, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 0XY, UK.
Science (New York, N.Y.)
|May 30, 2015
概括
一个前进的遗传屏幕确定了人类沉默枢纽 (HUSH) 复合体,对表观遗传抑制至关重要. 失去HUSH会降低H3K9me3,影响异性染色素和基因沉默.
科学领域:
- 表观遗传学和基因调控
- 人类分子遗传学 人类分子遗传学
背景情况:
- 对基因组稳定性和基因沉默而言,异色染色体的形成和维护至关重要.
- 在像Drosophila这样的模型生物中进行前进的遗传选在发现表观遗传调节者的过程中发挥了重要作用.
研究的目的:
- 通过前进基因选,识别人类细胞中涉及表观遗传抑制的新基因和复合体.
- 描述新发现的HUSH复合体在维持异性染色素中的功能.
主要方法:
- 在近平形KBM7人类细胞中进行了非致命的前进基因选.
- 分析H3K9me3水平在内生局部和综合逆转录病毒中,在失去HUSH组件时.
- 研究了HUSH和SETDB1对H3K9me3丰富基因组区域的招募.
主要成果:
- 包括TASOR,MPP8和周边蛋白在内的HUSH复合体被确定为人类细胞表观遗传抑制的关键.
- 失去HUSH组件导致H3K9me3水平在内源基因组位点和集成逆转录病毒中显著下降.
- 该HUSH复合体被招募到H3K9me3丰富的位置,促进SETDB1招募持续的异色素蛋白形成和转录沉默.
结论:
- 哈什复合体是人类细胞内表观遗传机制的保存,必不可少的组成部分,与多菌不同.
- 通过H3K9三甲基化,HUSH在建立和维持异染色素方面发挥着关键作用,从而调节基因表达.
相关概念视频
02:32Position-effect Variegation
7.3K
In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.
7.3K
01:37Epigenetic Regulation
4.3K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
4.3K
01:46Epigenetic Regulation
34.4K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
34.4K
01:46Epigenetic Regulation
26.3K
26.3K
02:35Chromatin Position Affects Gene Expression
25.3K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
25.3K
02:30Genomic Imprinting and Inheritance
38.9K
Diploid organisms inherit genetic material through chromosomes from both parents. Copies of the same gene are known as alleles. In most cases, both alleles are simultaneously expressed and allow various cellular processes to function optimally. If one of the alleles is missing or mutated, the expression of the other allele can compensate; however, this is not true for all genes.
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
The expression of some genes depends on which parent passed the gene to the offspring, through a phenomenon known as...
38.9K