通过选择性自来调节内细胞网膜周转
Aliaksandr Khaminets1, Theresa Heinrich2, Muriel Mari3
1Institute of Biochemistry II, Goethe University School of Medicine, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany.
FAM134蛋白质作为内细胞网膜 (ER) 受体,启动ER-phagy以维持细胞平衡. 这一过程对于ER形态,营业额和预防神经元退化至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 神经科学是一个神经科学.
背景情况:
- 细胞内膜网膜 (ER) 是蛋白质和脂质合成,离子稳态和细胞通信的重要器官.
- 自对于ER周转是必不可少的,但其对ER降解的调节机制仍然不清楚.
研究的目的:
- 阐明通过自 (ER-phagy) 来降解ER降解的调节机制.
- 确定FAM134蛋白在选择性ER循环和细胞平衡中的作用.
主要方法:
- 研究了FAM134网络蛋白作为与LC3和GABARAP自修饰剂结合的ER居民受体.
- 利用具有FAM134B下调或过度表达的人类细胞系来观察ER形态和降解.
- 产生Fam134b破坏的小鼠以评估体内ER周转率,应激反应和神经元完整性.
主要成果:
- FAM134蛋白直接与LC3/GABARAP结合,通过ER-phagy进行介导.
- 人体细胞中FAM134B缺乏导致ER扩张;过度表达导致碎片化和降解.
- 与人类感官神经病变相关的突变FAM134B损害了ER-phagy受体的功能.
- 在小鼠中Fam134b的破坏导致ER扩张,抑制循环,增加亡和感觉神经元退化.
结论:
- 由FAM134蛋白调节的选择性ER-phagy对于维持哺乳动物细胞稳态至关重要.
- FAM134蛋白质控制ER形态和循环,对于防止ER扩张和神经元细胞死亡至关重要.
更多相关视频
06:58Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles
Published on: October 18, 2024
09:34The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells
Published on: July 27, 2018
相关概念视频
Delivery Pathways to the Lysosome
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Export of Misfolded Proteins out of the ER
Autophagy
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Regulation of the Unfolded Protein Response
ER Retrieval Pathway
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
The Unfolded Protein Response
