N(6) -甲基氨酸调节信使RNA的翻译效率
Xiao Wang1, Boxuan Simen Zhao1, Ian A Roundtree2
1Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA; Howard Hughes Medical Institute, The University of Chicago, 929 East 57th Street, Chicago, IL 60637, USA.
Cell
|June 6, 2015
概括
在mRNA中的N(6) - 甲基氨酸 (m(6) A) 控制基因表达. 读者蛋白YTHDF1和YTHDF2调节mRNA稳定性和蛋白质合成,从而实现快速和可控的细胞反应.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 在RNA生物学,RNA生物学.
背景情况:
- 甲基氨酸 (m(6) 是哺乳动物信使RNA (mRNA) 中最常见的内部修饰.
- m(6) 修改是动态的,可逆的,在调节mRNA代谢中起着至关重要的作用.
- 这种表观遗传标记在转录后为基因表达增加了一层控制.
研究的目的:
- 阐明m(6)A读者蛋白在mRNA调节中的不同作用.
- 了解m(6) A介导的mRNA衰变和翻译之间的相互作用.
- 定义一种统一的机制来控制细胞质中基于m(6) A的基因表达.
主要方法:
- 研究了m(6)A读者蛋白的功能,特别是人类YTHDF1和YTHDF2.
- 分析了m(6)A修改对mRNA稳定性和翻译效率的影响.
- 描述了受m(6) A.A.影响的细胞质调节通路.
主要成果:
- 人类YTHDF2识别了m(6) A,并降低了目标mRNA转录的稳定性,控制了它们的寿命.
- 人类YTHDF1与翻译机制相互作用,从m(6) A-修饰的mRNA中积极促进蛋白质合成.
- 通过YTHDF2介导的降解和YTHDF1介导的翻译促进共同调节蛋白质的生产.
结论:
- m(6) 一种修饰提供了快速基因表达反应的机制.
- YTHDF1和YTHDF2的对立但协调的作用确保了从动态mRNA转录中可控制的蛋白质生产.
- 基于A的调控提供了一个复杂的系统,用于微调哺乳动物细胞中的蛋白质输出.
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