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结合体U4/U6.U5三snRNP的结构

Thi Hoang Duong Nguyen¹, Wojciech P Galej¹, Xiao-chen Bai¹

¹MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.

|June 25, 2015

概括

U4 / U6.U5 三snRNP 结合体结构揭示了RNA解和蛋白质组件如 Prp8 和 Brr2 如何激活关键基因表达的结合体. 这提供了关于结合体激活及其活性位点的见解.

科学领域:

分子生物学分子生物学
结构生物学结构生物学
遗传学遗传学是一个

背景情况:

拼接体是一个大型分子机器,对基因表达至关重要,催化了mRNA前拼接.
U4/U6.U5三snRNP是一个关键的预组合复合体,它经历了显著的重组,成为催化活性.
了解tri-snRNP的结构对于破译拼接酶激活机制至关重要.

研究的目的:

为了确定Saccharomyces cerevisiae U4/U6.U5 tri-snRNP.的高分辨率结构.
阐明RNA和蛋白质组件在tri-snRNP中的组织.
为了深入了解结合体激活的初始步骤和活跃部位的形成.

主要方法:

低温电子显微镜 (cryo-EM) 单颗粒重建.
高分辨率结构分析 (5.9 Å分辨率).

主要成果:

冷-EM结构揭示了三-snRNP的RNA和蛋白质组成部分的完整组织.
Brr2酶活性位点已准备好释放U4 / U6 snRNAs.
Prp8和Snu114将U5 snRNA循环I放置在Prp8活性部位腔内,为拼接对准前子.

结论:

确定的结构为结合体的激活机制提供了关键的见解.
这些发现揭示了结合体组合和催化作用的结构基础.
这项研究提供了一个详细的视图的spliceosome的活性部位的组件和他们的安排.

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