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Updated: Apr 8, 2026

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
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Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

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在超微微电极上观察单蛋白和DNA大分子碰撞

Jeffrey E Dick1, Christophe Renault1, Allen J Bard1

  • 1Center for Electrochemistry, Department of Chemistry, The University of Texas at Austin, Austin, Texas 78712, United States.

Journal of the American Chemical Society
|June 26, 2015
PubMed
概括

这项研究引入了一种新的电化学方法,用于检测单个生物大分子. 该技术利用电极位点阻断来识别单个酶,抗体和DNA,实现分析的最终灵敏度.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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