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Updated: Apr 8, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

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用于细胞内mRNA检测的FRET纳米火焰:避免假阳性信号并最大限度地减少系统波动的影响

Yanjing Yang1, Jin Huang1, Xiaohai Yang1

  • 1State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, P. R. China.

Journal of the American Chemical Society
|June 26, 2015
PubMed
概括

研究人员开发了新的光共振能量转移 (FRET) 纳米火焰,用于感知活细胞中的mRNA. 与旧方法相比,这些探测器通过减少假阳性和系统波动来提高准确性.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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