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Updated: Apr 7, 2026

Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
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Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice

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囊性口炎病毒L蛋白的结构来自电子冷显微镜

Bo Liang1, Zongli Li2, Simon Jenni3

  • 1Department of Microbiology and Immunobiology, Harvard Medical School, Boston, MA 20115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 20115, USA.

Cell
|July 7, 2015
PubMed
概括

研究人员使用电子冷显微镜确定了膀性口腔炎病毒 (VSV) 大 (L) 蛋白质的结构. 这揭示了病毒RNA依赖RNA聚合酶 (RdRp)及其相关的酶域的原子细节.

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Correlative Light Electron Microscopy CLEM for Tracking and Imaging Viral Protein Associated Structures in Cryo-immobilized Cells09:18

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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
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Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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