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加列-3结合蛋白和加列-1在乳腺癌细胞聚合和转移中的相互作用
Tzu-Wen Lin, Hui-Tzu Chang1, Chein-Hung Chen
1§Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei 112, Taiwan.
Journal of the American Chemical Society
|July 14, 2015
概括
加勒-3结合蛋白 (Gal-3BP) 与加勒-1的相互作用驱动癌细胞聚合和转移. 本研究详细介绍了参与这些关键癌细胞粘附过程的Gal-3BP上的特定N-甘氨酸结构.
科学领域:
- 葡萄糖生物学 葡萄糖生物学
- 癌症生物学 癌症生物学
- 质谱测量质量谱测量
背景情况:
- 加勒-3结合蛋白 (Gal-3BP) 是一种参与癌症转移的高糖化蛋白.
- 加尔-3BP通过加勒因相互作用调解瘤细胞聚合,增强循环中的癌细胞存活率.
- 与Gal-3BP相互作用的特定甲烯和它们的结合特异性仍然在很大程度上未被表征,这是由于糖测序的限制.
研究的目的:
- 为了阐明不同癌细胞系 (MCF-7和MDA-MB-231) 的Gal-3BP的N-甘氨酸结构.
- 为了识别与Gal-3BP相互作用的特定加勒素,并确定结合特异性.
- 了解Gal-3BP糖化在癌细胞聚合和转移中的作用.
主要方法:
- 液态染色体质谱法 (LC-MS) 用于分析Gal-3BP上的N-甘氨酸.
- 使用特定的外甘油酶反应来确定N-甘氨酸序列,包括终端化,化和LacNAc重复.
- 使用不同的癌症细胞系进行了加勒素相互作用测试.
主要成果:
- 从两种细胞系的Gal-3BP上鉴定出具有终端α2,3-酸和核的复杂型N-甘氨酸.
- MCF-7细胞表现出额外的α1,2-和α1,3-糖结合,而MDA-MB-231细胞显示出更少的四天线结构和更多的LacNAc重复.
- MDA-MB-231细胞利用表面的 galectin-1,而不是 galectin-3,与Gal-3BP相互作用,形成大型寡合物和细胞聚合物.
结论:
- 该研究确定了Gal-3BP上特定的N-甘氨酸结构,这些结构决定了其与加勒-1的相互作用.
- 加尔-3BP与加勒-1的相互作用对于调解癌细胞聚合和潜在的转移至关重要.
- 这项研究提供了对Gal-3BP - 黄素相互作用及其对癌症进展的影响的结构性见解.
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