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Single Particle Cryo-Electron Microscopy: From Sample to Structure

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纳米粒子成像单个纳米晶体的3D结构通过电子显微镜

Jungwon Park¹, Hans Elmlund², Peter Ercius³

¹Department of Chemistry, University of California, Berkeley, CA 94720, USA. Department of Applied Physics, Harvard University, Cambridge, MA 02138, USA. School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA.

Science (New York, N.Y.)

|July 18, 2015

概括

研究人员使用先进的电子显微镜开发了一种新的方法来确定溶液中的单个纳米粒子的3D结构. 这一突破允许在异质纳米粒子群体中进行纳米尺度缺陷分析.

科学领域:

纳米技术
材料科学
物理化学

背景情况:

了解体纳米粒子的合成,生长和特性受到技术限制的阻碍.
在高分辨率下对溶液中的单个纳米粒子进行描述仍然是一个挑战.

研究的目的:

引入一种用于确定溶液中单个纳米颗粒的三维 (3D) 结构的新方法.
克服纳米粒子表征的技术障碍.

主要方法:

使用石墨烯液体细胞与高分辨率传输电子显微镜相结合.
使用直接电子探测器和单粒子3D重建算法.

主要成果:

实现了单个纳米晶体的近原子分辨率3D结构.
证明分析溶液中自由旋转的纳米粒子的能力.

结论:

开发的方法可以研究异质和潜在无序的纳米粒子群体.
提供了解纳米级结构和溶液合成纳米粒子缺陷的稳定性的手段.

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