清除组织清除
Douglas S Richardson1, Jeff W Lichtman2
1Harvard Center for Biological Imaging, Harvard University, Cambridge, MA 02138, USA; Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Cell
|July 18, 2015
概括
通过减少光散射,组织清除技术可以对生物样本进行深度成像. 最近的创新和显微镜的进步正在改善三维组织的可视化.
科学领域:
- 生物成像
- 生物物理
- 显微镜
背景情况:
- 生物样本本质上是三维的.
- 光散射会阻碍深层组织的成像.
- 组织清除旨在减轻光线散射以增强可视化.
研究的目的:
- 在生物组织中检查光散射的物理原理.
- 描述各种组织清除技术背后的机制.
- 讨论最近的光显微镜成像清除组织的进展.
主要方法:
- 对光散射的物理原理进行回顾.
- 现有和新兴清算技术的机制描述.
- 清除样品的先进光显微镜方法的概述.
主要成果:
- 了解光散射对于深层组织成像至关重要.
- 各种清除方法 (例如,折射率匹配,脱水) 有效地减少光散射.
- 新的显微镜技术使得清除的大容量生物样本的高分辨率成像成为可能.
结论:
- 组织清除对于高分辨率,深层组织成像至关重要.
- 在清除和显微镜的近期创新显著提升了生物可视化.
- 这一综述为理解和应用这些技术提供了基础.
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