无线光流体系统用于可编程的体外药理学和光遗传学
Jae-Woong Jeong1, Jordan G McCall2, Gunchul Shin3
1Department of Electrical, Computer, and Energy Engineering, University of Colorado, Boulder, CO 80309, USA; Department of Materials Science and Engineering, Beckman Institute for Advanced Science and Technology and Frederick Seitz Materials Research Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
Cell
|July 21, 2015
概括
研究人员开发了无线光流体神经探针,用于微侵袭性体内研究. 这些先进的探测器能够精确地控制自由行为动物的药物输送和光刺激.
科学领域:
- 神经科学
- 生物医学工程
- 生物仪器
背景情况:
- 在体内药理学和光遗传学对于了解动物的神经回路和系统至关重要.
- 目前的神经接口技术 (金属管,连接光纤) 限制了最小侵入性,无连接的研究.
- 需要先进的工具来准确无线控制神经生理操作.
研究的目的:
- 引入新的无线光流体神经探针,结合微流体药物输送和微型LED阵列.
- 为了证明无线的空间时间控制流体输送和光刺激的能力.
- 展示这些探针在操纵自由行为动物的神经生理系统中的应用.
主要方法:
- 与细胞规模无机发光二极管 (μ-ILED) 阵列集成的超薄软微流体通道的开发.
- 无线,编程控制流体输送和基于光的刺激.
- 在自由移动的动物体内测试基因表达修饰,联体输送和行为操纵.
主要成果:
- 无线空间时间控制药物输送和光刺激的成功演示.
- 在体内基因表达的修改和配体的输送.
- 通过并发光刺激和对抗药物递送来操纵与奖励相关的表现.
结论:
- 无线光流体神经探测器为先进的体内神经科学研究提供了极少入侵的解决方案.
- 这些设备提供了前所未有的控制神经电路和细胞信号操纵.
- 该技术在各种器官系统,物种和生物医学领域都有广泛的潜在应用.
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