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在DNA表面附近的异常快速水分扩散动力学

John M Franck^1,2, Yuan Ding³, Katherine Stone^1,4

¹Department of Chemistry and Biochemistry, University of California , Santa Barbara, California 93106, United States.

Journal of the American Chemical Society

|August 11, 2015

概括

透过Overhauser效应的动态核极化 (ODNP) 发现DNA表面的水异常快速移动,与蛋白质或膜附近的水不同. 这种快速移动的水可能会降低DNA相互作用的激活障碍.

科学领域:

生物物理
化学物理
分子生物学

背景情况:

在Overhauser效应的动态核极化 (ODNP) 技术探测水在表面附近的移动性.
了解DNA表面的水动力学对于分子相互作用至关重要.

研究的目的:

扩大ODNP在DNA表面测量水动态的能力.
在DNA复合体附近研究水的转化流动性.

主要方法:

将氧化基 (旋转标签) 附着在DNA酸盐骨干上.
应用一种新的ODNP分析来区分水种群.
进行与绑定DNA和改变氧化物流动性的对照研究.

主要成果:

在DNA表面附近的大量水体表现出快速,大规模的转化扩散.
这种移动水在蛋白质或膜表面附近的移动速度比水更快.
DNA 表面与其水化水网的很大一部分相互作用.

结论:

与其他生物分子表面相比,DNA表面促进了异常快速的水扩散.
与表面水的弱相互作用可以降低DNA介导相互作用的激活障碍.
这一发现为DNA在生物过程中的作用提供了新的见解.

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