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使用高通量成像绘图识别基因定位因素

Sigal Shachar¹, Ty C Voss², Gianluca Pegoraro²

¹National Cancer Institute, NIH, Bethesda, MD 20892, USA.

|August 16, 2015

概括

我们开发了HIPMap,一个高通量成像管道, 这种方法确定了50个对于保持正确的空间基因组定位至关重要的新因素.

科学领域:

细胞生物学
基因组学
分子生物学

背景情况:

基因组在细胞核的3D空间中是非随机的.
了解基因组组织是细胞功能的关键.

研究的目的:

为绘制基因组区域位置开发高通量方法.
确定涉及空间基因组组织的细胞因素.

主要方法:

开发了HIPMap (高精度,高通量,自动化光现场混合成像管道).
使用HIPMap对人类细胞进行无偏的siRNA查.
绘制多种基因组位置的空间位置.

主要成果:

确定了50个细胞因子,
定位因素包括染色体重塑剂,基因组修饰剂和核蛋白.
复制和染色体重组机械组件是关键的定位因素.
在基因定位方面,细胞循环的通过复制而非线粒分裂的进展至关重要.

结论:

HIPMap提供了一种用于绘制基因组位置的大规模方法.
确定了涉及空间基因组组织的细胞因子汇编.
这项研究提升了我们对基因组架构背后的分子机制的理解.

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