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菌类诱导的细胞混合是竞争性组织入侵和破坏所必需的
Romain Levayer1, Barbara Hauert1, Eduardo Moreno1
1Institute for Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland.
Nature
|August 20, 2015
概括
基因菌驱动细胞竞争,促进组织入侵. 细胞间和改变的细胞结合是这一过程中的关键机制,将癌基因活动与病变组织变化联系起来.
科学领域:
- 发育生物学
- 细胞生物学
- 癌症学
背景情况:
- 在发育过程中,细胞间的间接形成组织.
- 细胞竞争消除了不理想的细胞,保持组织健康.
- 在癌症等病理中, 介质的作用尚不清楚.
研究的目的:
- 研究原基因菌在细胞竞争中的作用.
- 确定细胞间是否有助于瘤基因诱导的组织扩张.
- 阐明了竞争期间失败细胞消除的机制.
主要方法:
- 在Drosophila幼和翅膀形象盘中进行长期活体成像.
- 细胞与细胞接触,接口形态和细胞混合的分析.
- 研究差异生长,表面张力,F-actin和酸 (3,4,5) -三酸盐的作用.
主要成果:
- 输家细胞消除的概率与赢家-输家接触面积相关.
- 细胞间的合促使获胜者与失败者的细胞混合,
- 通过F-actin和PIP3调节的差异界面张力促进细胞混合并降低克隆的紧性.
结论:
- 建立了原生瘤菌,细胞间和瘤样扩张之间的联系.
- 证明通过化进行细胞混合是损失细胞消除的必要条件.
- 揭示了变化的界面张力动力驱动瘤基因诱导的细胞竞争和组织侵入性.
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