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在可见光控制的蛋白质上形成亚桥

Christian Hoppmann¹, Innokentiy Maslennikov², Senyon Choe²

¹Department of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California San Francisco , San Francisco, California 94158, United States.

Journal of the American Chemical Society

|August 25, 2015

概括

研究人员开发了一种新的小分子光开关F-PSCaa, 这种可见光控制的光开关可以在光学生物学中精确的光学调制蛋白质功能.

科学领域:

生物化学
分子生物学
视觉遗传学

背景情况:

生物过程的光学调制提供了高的时空分辨率.
基于蛋白质的光开关是有价值的,
小分子光开关可以提供特异性等优势,但缺乏遗传编码性和可见光响应性.

研究的目的:

开发一个具有遗传编码能力和可见光控制的小分子光开关.
创建一个在位蛋白质调节的工具,

主要方法:

设计了一种基于五酸的可光切换的点击氨基酸 (F-PSCaa).
用扩展的遗传代码将F-PSCaa转化为蛋白质.
在现场诱导的桥形成和可切换光的形状变化.

主要成果:

在暴露于可见光时,F-PSCaa异构.
基因内置的F-PSCaa与附近的半氨酸形成了局部基桥梁.
可见光成功调节了蛋白质构成和卡尔莫杜林结合.

结论:

开发了一种对可见光有反应的基因编码小分子光开关.
在光学应用中,F-PSCaa光学开关提供了位置灵活性和最小干扰.
这种工具使用生物相容的可见光进行了蛋白质功能的光学控制.

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