作为广泛保护性免疫原的一种稳定的三元性流感血凝素干
Antonietta Impagliazzo1, Fin Milder2, Harmjan Kuipers2
1Crucell Vaccine Institute, Janssen Center of Excellence for Immunoprophylaxis, Archimedesweg 4-6, 2301 CA Leiden, Netherlands. aimpagli@its.jnj.com wilson@scripps.edu.
Science (New York, N.Y.)
|August 26, 2015
概括
研究人员设计出稳定的血凝素干抗原 (迷你HA),产生广泛中和抗体 (bnAbs). 通过模仿关键的HA干结构,这种方法显示出普遍流感疫苗的前景.
科学领域:
- 免疫学
- 疫苗学
- 结构生物学
背景情况:
- 针对流感血凝素 (HA) 干的广泛中和抗体 (bnAbs) 提供了通用流感疫苗的潜力.
- 开发稳定的HA干抗原对于产生有效的bnAbs至关重要.
研究的目的:
- 设计稳定的HA干模仿物 (迷你HA) 能够产生bnAbs对抗A型流感病毒.
- 评估设计的小型HA的免疫性和保护性.
主要方法:
- 基于H1亚型的合理设计和库方法来设计稳定的HA干抗原 (迷你HA).
- 结构和结合测试,以比较小HA与全长HA.
- 在小鼠和非人类灵长类动物中进行临床前挑战性研究.
主要成果:
- 最先进的迷你HA候选物表现出与全长HA相似的结构和bnAb结合特性.
- 在小鼠中,Mini- HA对致命的异型和异型亚型流感具有完全的保护作用.
- 由小HA引起的抗体中和了多种HA,与人类bnAbs竞争,并显示了效应器功能.
结论:
- 设计的HA干模仿物 (迷你HA) 可以产生广泛的中和抗体.
- 这项研究提供了基于HA干抗原开发通用流感疫苗的概念证明.
更多相关视频
07:55Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
Published on: August 29, 2017
12.3K
12:18Expression of Functional Recombinant Hemagglutinin and Neuraminidase Proteins from the Novel H7N9 Influenza Virus Using the Baculovirus Expression System
Published on: November 6, 2013
25.0K
相关概念视频
00:42Cross-reactivity
33.9K
Overview
33.9K
01:27Influenza
42
Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...
42
02:28Leaky Scanning
5.9K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.9K