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相关概念视频

Buffers02:56

Buffers

177.6K
A solution containing appreciable amounts of a weak conjugate acid-base pair is called a buffer solution, or a buffer. Buffer solutions resist a change in pH when small amounts of a strong acid or a strong base are added. A solution of acetic acid and sodium acetate is an example of a buffer that consists of a weak acid and its salt: CH3COOH (aq) + CH3COONa (aq). An example of a buffer that consists of a weak base and its salt is a solution of ammonia and ammonium chloride: NH3 (aq) + NH4Cl...
177.6K
Phosphate Buffer01:22

Phosphate Buffer

6.1K
The phosphate buffer system is a critical biological mechanism for maintaining pH stability in the body. This system operates primarily through two components: sodium dihydrogen phosphate (NaH2PO4), which acts as a weak acid, and sodium hydrogen phosphate (Na2HPO4), which serves as a weak base.
Sodium dihydrogen phosphate does not fully dissociate in neutral or acidic solutions. When a strong base, such as sodium hydroxide (NaOH), is introduced into the solution, sodium dihydrogen phosphate...
6.1K
Photoreceptors and Visual Pathways01:22

Photoreceptors and Visual Pathways

11.1K
At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category,...
11.1K
Buffers: Overview01:30

Buffers: Overview

11.0K
Buffers play a crucial role in stabilizing the pH of a solution by mitigating the effects of small amounts of added acid or base. They consist of a weak acid and its conjugate base or a weak base and its conjugate acid. A solution of acetic acid and sodium acetate is an example of a buffer that consists of a weak acid and its salt: CH3COOH (aq) + CH3COONa (aq). An example of a buffer that consists of a weak base and its salt is a solution of ammonia and ammonium chloride: NH3 (aq) + NH4Cl (aq).
11.0K
Polyprotic Acids03:38

Polyprotic Acids

34.6K
Acids are classified by the number of protons per molecule that they can give up in a reaction. Acids such as HCl, HNO3, and HCN that contain one ionizable hydrogen atom in each molecule are called monoprotic acids. Their reactions with water are:
34.6K

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相关实验视频

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Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5&#8242;-Phosphate
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Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate

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在可见光下在PBS缓冲器中的可逆光酸功能

Nawodi Abeyrathna1, Yi Liao1

  • 1Department of Chemistry, Florida Institute of Technology , Melbourne, Florida 32901, United States.

Journal of the American Chemical Society
|August 26, 2015
PubMed
概括

在可见光下可逆释放质子. 这种光激活分子在生物环境中具有控制质子转移的潜力.

科学领域:

  • 摄影化学
  • 超分子化学
  • 化学生物学

背景情况:

  • 质子转移对许多生物过程至关重要.
  • 在生理条件下,动态控制质子度 (pH) 是一个挑战.
  • 光反应分子提供对化学反应的外部控制.

研究的目的:

  • 在可见光下设计和描述能够反向释放质子的光酸.
  • 在生物相关的缓冲剂 (PBS,pH 7.4) 中评估其性能.
  • 在生物医学领域探索其潜在应用.

主要方法:

  • 设计基于因达的双酸性质和分体化.
  • 使用可见光照射进行光反应研究.
  • 量子产量确定
  • 在水溶液中的pH值测量.

主要成果:

  • 一种超稳定状态的光酸已成功合成.
  • 在可见光下在PBS缓冲剂 (pH 7. 4) 中实现了可逆质子释放.
  • 在中等光强度 (10^2 μmol·m−2·s−1) 时的高量子产量 (0.73) 和效率.
  • 在0.1mM水溶液中显示出1. 7单位的可逆pH变化.

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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

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An Integrated System to Remotely Trigger Intracellular Signal Transduction by Upconversion Nanoparticle-mediated Kinase Photoactivation
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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells
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Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

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结论:

  • 开发的光酸在可见光下提供有效和可逆的质子控制.
  • 它的特性适用于生理条件,可用于控制质子转移过程.
  • 这种光酸对于需要精确的pH调节的生物医学应用来说是一个有前途的工具.