整合剂介导增强性RNA的生物发生
Fan Lai1, Alessandro Gardini1, Anda Zhang1
1University of Miami Miller School of Medicine, Sylvester Comprehensive Cancer Center, Department of Human Genetics, Biomedical Research Building, Room 719, 1501 NW 10th Avenue, Miami, Florida 33136, USA.
Nature
|August 27, 2015
概括
整合体对增强型RNA (eRNA) 的生物发生和功能至关重要. 它处理eRNA转录,从而实现基因表达的调节.
科学领域:
- 分子生物学
- 基因调控
- 表观遗传学
背景情况:
- 整合剂复合体与RNA聚合酶II (RNAPII) 相关,并具有内核酶活性.
- 这种活动对于处理特定的小核RNA基因至关重要.
- 它在增强器RNA (eRNA) 生物发生和转生动物中的功能中的作用仍然在很大程度上未被探索.
研究的目的:
- 研究整合器复合体在增强性RNA (eRNA) 的生物发生中的作用.
- 确定整合剂在刺激依赖的增强剂活性和基因调节中的参与.
- 阐明整合器影响eRNA处理和转录终止的机制.
主要方法:
- 调查集成者招募到增强器和超级增强器.
- 使用了整合器子单元的功能耗尽.
- 进行了全球核试验和RNAPII分析.
- 评估了对eRNA诱导,染色质循环和基因表达的影响.
主要成果:
- 整合器以一种依赖刺激的方式被招募到增强剂中.
- 整合剂的耗尽减少了eRNA的诱导,并废除了增强剂-促进剂循环.
- 需要整合器来进行eRNA转录的3'-end切割,以促进转录终止.
- 整合剂的催化活性对于eRNA诱导和基因表达反应至关重要.
结论:
- 整合剂在增强性RNA (eRNA) 的生物发生中起着至关重要的作用.
- 整合器通过调节eRNA处理和转录终止来促进增强器功能.
- 整合剂的催化活性对于刺激诱导的基因表达是不可或缺的.
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