富含血小板血对急性裂阿基里斯肌愈合组织的影响:人类免疫组织化学研究
Joseph Alsousou1, Mark Thompson2, Paul Harrison3
1University of Liverpool, Liverpool, UK.
Lancet (London, England)
|August 28, 2015
概括
通过增加原I和糖氨基酸含量,富血小板血 (PRP) 治疗改善了破裂的阿基里斯的愈合. 这项研究提供了PRP的第一个体内免疫组织化学证据.
科学领域:
- 整形医学
- 复原医学
- 生物材料科学
背景情况:
- 富血小板血 (PRP) 含有缩的血小板,促进组织愈合.
- 实验室和动物研究表明PRP有助于骨,软骨,肌和肌肉的愈合.
- 没有先前的人体体内研究研究了PRP对肌愈合的影响.
研究的目的:
- 用PRP治疗的人类阿基里斯破裂的免疫组织化学反应.
- 评估PRP对愈合肌的细胞性,原沉积,血管性和糖氨基酸含量的影响.
主要方法:
- 在治疗后6周的20名患者的阿基里斯肌超声指导活检 (10个PRP,10个安慰剂).
- 使用H&E和Alcian蓝色染色的组织学分析.
- 用图像J分析检测I,III,CD45,KI67和CD34的免疫组织化学.
主要成果:
- 接受PRP治疗的肌显著增加了细胞性,糖氨基甘氨酸和原I含量.
- 在PRP样本中观察到纤维结构的改善和较低的III原与I原比率.
- PRP治疗导致血管性降低 (CD34) 和改善早期愈合得分 (修改后的Bonar).
结论:
- 在人体中,局部应用PRP可提高阿基里斯肌的成熟度.
- PRP促进了较高的 I 型原沉积,减少了细胞性和血管性,并增加了葡萄糖氨基酸.
- 需要进一步研究以确定PRP在肌肉骨疾病中的长期影响.
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