对酒精脱酶的急性酒精性肝炎和细胞Th1免疫反应
Laura J Blackmore1, Jennifer M Ryan1, Xiaohong Huang1
1Institute of Liver Studies & Transplantation, King's College London, London, UK.
Lancet (London, England)
|August 28, 2015
概括
与肝硬化患者相比,酒精性肝炎患者对酒精脱酶 (ADH) 呈现免疫反应,具有更强的促炎T助手1型 (Th1) 反应. 这表明Th1免疫抑制是酒精性肝炎的潜在治疗方法.
科学领域:
- 免疫学
- 肝病学
- 胃肠病学
背景情况:
- 酒精性肝炎是一种严重的肝炎,死亡率高.
- 之前的研究表明酒精脱酶 (ADH) 特定的免疫反应与酒精相关的肝硬化.
- 在酒精性肝炎中存在ADH特异性细胞免疫仍未确定.
研究的目的:
- 确定酒精性肝炎患者是否存在ADH特异性细胞免疫.
- 将酒精性肝炎的免疫反应与酒精相关肝硬化和健康对照的免疫反应进行比较.
主要方法:
- 从15名酒精性肝炎患者,9名肝硬化患者和3名健康对照患者收集了周围血液单核细胞 (PBMC).
- 通过 (3H) - 提米丁的结合评估了人类ADHβ1亚单元的25个重叠的PBMC增殖.
- 使用ELISA测定了干扰素 (IFNγ),干扰素-17 (IL-17) 和IL-4的水平.
主要成果:
- 在15名酒精性肝炎患者中,有7人对ADH产生增殖反应.
- 在酒精性肝炎患者的PBMC中检测到促炎细胞因子IFNγ和IL-17.
- 虽然肝硬化患者也表现出ADH的识别,但酒精性肝炎患者表现出更明显的Th1 (IFNγ) 和Th17 (IL-17) 反应.
结论:
- 在酒精性肝炎中,增殖性抗ADH免疫反应针对特定的表位区域.
- 酒精性肝炎的特征是具有主导性的促炎T助手1型 (Th1) 免疫反应.
- 旨在抑制Th1免疫力的向疗法应用于治疗酒精性肝炎.
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