这是一个很好的例子. 扩展分辨率的内细胞和细胞骨动态结构化照明成像
Dong Li1, Lin Shao1, Bi-Chang Chen1
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Science (New York, N.Y.)
|August 29, 2015
概括
我们提高了活细胞的超高分辨率显微镜, 以达到更高分辨率观察蛋白质动态. 这种先进的成像揭示了前所未有的细节细胞结构和过程.
科学领域:
- 细胞和分子成像
- 生物物理
- 纳米技术
背景情况:
- 超高分辨率光显微镜可以对活细胞中的蛋白质动态进行成像.
- 结构化照明显微镜 (SIM) 提供速度和低光,但分辨率增加有限 (2x).
研究的目的:
- 扩展活细胞SIM的分辨率,以详细的图像细胞动态.
- 应用增强的SIM技术来可视化特定的蛋白质组合和器官.
主要方法:
- 开发了超高数值孔径SIM,用于84nm的侧面分辨率.
- 实现了45-62nm分辨率的非线性SIM与模式激活.
- 应用这些方法对活细胞动态的多色成像.
主要成果:
- 实现活细胞动态的高分辨率 (84nm和45-62nm) 多色成像.
- 视觉化了克拉特林和卡韦林组合,内体中的Rab5a和α-actinin.
- 在3D中检查了线粒体,行为体和戈尔吉器官的动态.
结论:
- 改进的SIM技术显著提高了活细胞成像的分辨率.
- 这些方法可以详细观察蛋白质动态和器官功能.
- 提供纳米级细胞过程的新见解.
更多相关视频
10:51Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
Published on: April 16, 2014
9.5K
12:44Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy f3D-SIM
Published on: September 29, 2014
20.5K
相关概念视频
01:28Three-Dimensional Microscopy in Microbiology
1.0K
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
1.0K
01:37Super-resolution Fluorescence Microscopy
14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
01:17Studying the Cytoskeleton
10.5K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
10.5K