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细菌糖结合物的化学酶组合用于特定地点的正交标签

Vinita Lukose¹, Garrett Whitworth¹, Ziqiang Guan²

¹Departments of Biology and Chemistry, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139, United States.

Journal of the American Chemical Society

|September 10, 2015

概括

这项研究使用化学酶方法探索细菌细胞表面的糖. 来自Campylobacter jejuni的修饰型糖结合物用于探测宿主-病原体相互作用.

科学领域:

微生物学
葡萄糖生物学
化学生物学

背景情况:

细菌细胞表面的糖对宿主-病原体相互作用至关重要.
了解这些相互作用需要化学定义和可修改的糖.
坎皮洛巴克特 (Campylobacter jejuni) 具有复杂的N链接蛋白质糖化 (Pgl) 途径.

研究的目的:

评估Campylobacter jejuni Pgl通路酶对化物修饰的UDP糖基质的耐受性.
开发化学酶方法来制备化物功能化甘氨酸和甘氨酸.
为研究C. jejuni糖联体在宿主殖民中的作用创建工具.

主要方法:

亚胺基改性UDP糖基质的化学酶合成.
使用Campylobacter jejuni Pgl通路酶进行体外酶测定.
无二酸盐结合的甘氨酸和糖的制备,含有特定位点的化物.

主要成果:

坎皮洛巴克特 (Campylobacter jejuni) Pgl酶可以耐受亚化物修饰的UDP糖基质.
化学酶方法使得特定位点的亚引入到肝糖糖体中.
成功合成了与非二酸盐结合的甘氨酸和具有亚胺功能的甘氨酸.

结论:

使用Campylobacter jejuni糖化酶可以合成亚胺基基结合物.
这些修改后的糖结合物作为研究细菌相互作用的有价值的工具.
开发的方法可以为探测生物功能提供直角标记.

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