在单个人类细胞中核膜相互作用的全基因组图
Jop Kind1, Ludo Pagie2, Sandra S de Vries2
1Division of Gene Regulation, Netherlands Cancer Institute, Plesmanlaan 121, 1066CX Amsterdam, the Netherlands; Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences) and University Medical Center Utrecht, Cancer Genomics Netherlands, Uppsalalaan 8, 3584CT Utrecht, the Netherlands.
Cell
|September 15, 2015
概括
哺乳动物的染色体与核膜形成膜相关域 (LAD). 新的单细胞映射揭示了一致的,基因贫乏的LAD和可变的,细胞类型特定的LAD,突出显示了染色体组织原理.
科学领域:
- 细胞生物学
- 基因组学
- 表观遗传学
背景情况:
- 哺乳动物的相间染色体通过膜相关域 (LAD) 与核膜 (NL) 相互作用.
- 了解单细胞中这些NL接触的全基因组结构对于阐明色素组织至关重要.
研究的目的:
- 开发和应用一种在人类单个细胞中绘制基因组范围内的核膜接触的方法.
- 描述单细胞LAD的一致性,可变性和协调性.
主要方法:
- 将近400个单个人类细胞中的核膜接触的全基因组映射.
- 分析LAD细胞对细胞的一致性,性敏感性以及与基因活性和基因素修饰的相关性.
主要成果:
- 确定了一致的基因贫乏的LAD和可变的细胞类型特定的LAD的核心架构.
- NL接触涉及多价值物相互作用和广泛的染色体内协调.
- LAD的一致性与基因活性相反,与H3K9me3正相关.
结论:
- 确定了单细胞染色体组织的基本原理.
- 证明了人类细胞中膜相关域的动态和协调性.
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