单细胞活动显示在甲类组合中直接的电子转移
Shawn E McGlynn1, Grayson L Chadwick1, Christopher P Kempes2,3,4
1Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, California 91125, USA.
Nature
|September 17, 2015
概括
微生物联盟使用直接的电子转移来进行合成合,而不仅仅是分子交换. 这一发现挑战了甲氧化古生物和硫酸盐减少细菌之间代谢相互作用的传统模型.
科学领域:
- 微生物学
- 生物地质化学
- 生物物理
背景情况:
- 微生物的多细胞组合促进了代谢合,使其具有挑战性的反应.
- 甲氧化古生物和硫酸盐降解细菌联盟是无氧甲氧化的关键.
- 这些协会中的代谢合机制尚不清楚.
研究的目的:
- 研究空间定位对未培养的甲氧化微生物群体的生物合成活动的影响.
- 阐明合成古生物和细菌之间的代谢相互作用和合机制.
- 挑战传统的缩模式.
主要方法:
- 在单个古生物和细菌细胞中测量稳定同位素的结合.
- 对物种间空间定位和细胞活动的分析.
- 基因组分析多血红细胞和矩阵染色.
主要成果:
- 细胞活动独立于物种交融和距离.
- 一个电导率模型最好地解释了经验数据.
- 通过多血红细胞和矩阵氧化还原活性发现了直接电子转移的证据.
结论:
- 在这些微生物联盟中,通过直接的物种间电子转移 (DIET) 发生了合成合.
- 这挑战了仅仅依赖于分子中间交换的合成模式.
- DIET为甲循环和其他生物化学过程中的微生物相互作用提供了新的视角.
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