生物聚合物的聚合溶液中的关联相互作用抵消了枯竭效应
Joost Groen1, David Foschepoth1, Esra te Brinke1
1Institute for Molecules and Materials, Radboud University , 6525 AJ, Nijmegen, The Netherlands.
Journal of the American Chemical Society
|September 19, 2015
概括
大肠杆菌细胞溶液中的宏分子拥挤涉及复杂的相互作用. 关联力抵消了小分子的耗尽,影响了基于大小和相互作用的整体效应.
科学领域:
- 生物物理
- 细胞生物学
- 生物化学
背景情况:
- 大肠杆菌细胞溶液是一个拥挤的环境,生物聚合物占据了体积的20-30%.
- 大分子的高度会产生消耗力,促进复合.
- 非特定的关联相互作用可以抵消枯竭,在细胞质中产生不清晰的平衡.
研究的目的:
- 在拥挤的生物环境中研究枯竭和关联性相互作用之间的平衡.
- 了解大分子拥挤如何影响大肠杆菌细胞质中的复合.
- 确定宏分子大小和关联性质在拥挤效应中的作用.
主要方法:
- 使用基于Förster共振能量转移 (FRET) 的探头在体外研究耗尽.
- 在各种拥挤的环境中检查消耗,包括合成拥挤剂和大肠杆菌溶解物.
- 研究了FtsZ原纤维的捆绑形成,作为更大规模枯竭相互作用的探测器.
主要成果:
- 在合成拥挤剂中,FRET探测器采用了更紧的构造,表明了强烈的耗尽.
- 在大肠杆菌溶解物和其他蛋白质拥挤剂中,消耗效应被否定,探针显示体积占用增加.
- 在合成拥挤剂和蛋白质溶液中,FtsZ原纤维束的形成发生了类似的情况.
结论:
- 在拥挤的生物聚合物溶液中,关联相互作用抵消了小宏分子的耗尽力.
- 大分子拥挤的净效应取决于大分子的大小及其与背景的关联相互作用.
- 了解这些对立的力量对于理解细胞组织和在拥挤的环境中的功能至关重要.
相关概念视频
Noncovalent Attractions in Biomolecules
20.4K
20.4K
Noncovalent Attractions in Biomolecules
66.1K
Noncovalent attractions are associations within and between molecules that influence the shape and structural stability of complexes. These interactions differ from covalent bonding in that they do not involve sharing of electrons.
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
Four types of noncovalent interactions are hydrogen bonds, van der Waals forces, ionic bonds, and hydrophobic interactions.
Hydrogen bonding results from the electrostatic attraction of a hydrogen atom covalently bonded to a strong-electronegative atom like oxygen,...
66.1K
Cooperative Allosteric Transitions
9.4K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
9.4K
Microbial Interactions: Competition
54
Microbial competition is an ecological interaction in which microorganisms vie for limited resources within shared environments. These resources may include nutrients, space, or light, depending on the system. The intensity and outcome of competition are influenced by the environmental context, such as nutrient availability, spatial constraints, and the diversity of microbial species present. These competitive interactions significantly influence the structure, function, and resilience of...
54
ATP and Macromolecule Synthesis
7.3K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
7.3K
Hydrolysis
124.7K
Overview
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
Hydrolysis is a chemical reaction in which the addition of water breaks down a polymer into its simpler monomer units. For example, peptides break into amino acids, carbohydrates into simple sugars, and DNA into nucleotides. Enzymes often facilitate these processes.
Hydrolysis Reverses Dehydration Synthesis
Complex carbohydrates can be broken down by breaking the bonds between individual sugar units. The reaction breaks a glycosidic bond as water is added to the compound. The...
124.7K


