通过连续的,由醇介导的自我传播反应,将分子检测事件自主转化为其光学性质的宏观变化
Hemakesh Mohapatra1, Hyungwoo Kim1, Scott T Phillips1
1Department of Chemistry, Pennsylvania State University , University Park, Pennsylvania 16802, United States.
Journal of the American Chemical Society
|September 22, 2015
概括
这项研究呈现了一种可检测醇并变得光的聚合物薄膜. 反应速率表明醇度,而光强度保持不变,显示出放大检测系统.
科学领域:
- 聚合物化学
- 化学传感器
- 材料科学
背景情况:
- 开发敏感和特定的化学检测方法对于各种应用至关重要.
- 现有的方法可能缺乏放大或定量信号响应.
- 响应性聚合物薄膜为集成传感平台提供了潜力.
研究的目的:
- 开发一种化学响应的聚合物薄膜来检测硫醇.
- 为了研究信号放大的一种自我传播反应.
- 为了区分放大和非放大光反应.
主要方法:
- 能够检测硫醇的功能化聚合物薄膜的制造.
- 在暴露于醇时启动自我传播反应.
- 测量光强度和反应速度.
- 与缺乏放大功能的控制膜进行比较.
主要成果:
- 聚合物薄膜检测到低水平的硫醇,并成为全球光.
- 光强度独立于放大系统中的二醇度.
- 光发育的速度与硫醇度成正比.
- 对照膜显示光强度与醇度成正比,总体强度较低.
结论:
- 开发的聚合物薄膜提供了放大,自我传播的光信号来检测醇.
- 该系统允许在反应速度与分析物度相关的情况下进行敏感检测.
- 与非放大方法相比,这种方法在信号放大方面提供了显著的改善.
相关概念视频
03:09Measuring Reaction Rates
23.5K
Polarimetry finds application in chemical kinetics to measure the concentration and reaction kinetics of optically active substances during a chemical reaction. Optically active substances have the capability of rotating the plane of polarization of linearly polarized light passing through them—a feature called optical rotation. Optical activity is attributed to the molecular structure of substances. Normal monochromatic light is unpolarized and possesses oscillations of the electrical...
23.5K
01:19Protein Dynamics in Living Cells
1.9K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
1.9K