通过微生物粘附到肠上皮细胞的Th17细胞诱导
Koji Atarashi1, Takeshi Tanoue2, Minoru Ando3
1RIKEN Center for Integrative Medical Sciences (IMS), 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; Department of Microbiology and Immunology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Cell
|September 29, 2015
概括
微生物对肠上皮细胞的粘附是诱导T辅助细胞17 (Th17) 的关键. 这种宿主特异性相互作用会影响肠道的免疫反应.
科学领域:
- 免疫学
- 微生物学
- 胃肠病学
背景情况:
- 肠道T辅助细胞17 (Th17) 对于宿主防御至关重要,并由特定的肠道微生物诱导.
- 分段细丝细菌 (SFB) 和某些病原体是已知的Th17细胞诱导体.
研究的目的:
- 研究微生物对肠上皮细胞 (ECs) 的粘附在 Th17 细胞诱导中的作用.
- 确定微生物粘附中的宿主特异性与Th17细胞诱导中的宿主特异性是否相关.
主要方法:
- 具有宿主特异性SFB (M-SFB和R-SFB) 的无菌小鼠和老鼠的单一殖民.
- 使用Citrobacter rodentium和Escherichia coli O157的感染模型,包括附着缺陷突变.
- 对微生物殖民和感染的Th17细胞诱导的分析.
主要成果:
- SFB对小肠EC表现出宿主特异性的粘附,导致宿主特异性的Th17细胞诱导.
- 在C. rodentium和E. coli O157的粘附缺陷突变中,未能诱导强大的Th17反应.
- 从性结肠炎患者中分离出来的20种细菌菌株的混合物,已知诱导Th17细胞,也显示出EC粘合性.
结论:
- 微生物对肠上皮细胞的粘附是Th17细胞诱导的关键启动信号.
- 主体特异性粘附机制可能是Th17细胞反应的主体特异性诱导的基础.
- 具有EC粘合性是强烈诱导Th17细胞的微生物的共同特征.
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