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Updated: Apr 1, 2026

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
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丹德里默细胞进入和贩运的时间解析蛋白质可视化

Linna Wang1, Li Yang1, Li Pan2

  • 1Department of Biochemistry, Purdue University, West Lafayette, IN, 47907, United States.

Journal of the American Chemical Society
|October 2, 2015
PubMed
概括

一个新的蛋白质组策略,TITAN,揭示了809种蛋白质在细胞进入过程中与树枝状体相互作用. 这种方法可以实时跟踪蛋白质-体相互作用,识别体介导和洞穴/体介导内细胞分裂的作用.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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