一个与ras相关的基因,具有转化抑制活性
H Kitayama1, Y Sugimoto, T Matsuzaki
1Laboratory of Molecular Oncology, Tsukuba Life Science Center, Ibaraki, Japan.
Cell
|January 13, 1989
概括
研究人员确定了Krev-1基因,该基因抑制癌细胞生长,与ras蛋白具有相似之处. 这种基因在各个物种中都存在,并且广泛表达,这表明它在组织调节中起着至关重要的作用.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 遗传学 是一个遗传学.
背景情况:
- 基斯肉瘤病毒对NIH/3T3细胞的转化导致恶性表型.
- 识别能够逆转这种转变的基因对于理解癌症生物学至关重要.
研究的目的:
- 单独和表征一种具有逆转NIH/3T3细胞转化表型的能力cDNA克隆.
- 调查已识别的基因的功能和进化保存.
主要方法:
- 从人类纤维细胞中分离一个cDNA表达库.
- 用Krev-1cDNA克隆感染NIH/3T3细胞.
- 对mRNA水平和表型抑制的分析.
- 与已知的蛋白质进行氨基酸序列比较.
- 南方斑分析以检测其他物种的同源序列.
主要成果:
- 一个1.8kb的cDNA克隆Krev-1被分离出来,并显示出诱导复原体的活性.
- 克雷夫-1转染剂显示了特定的mRNA水平与转化表型的抑制之间的相关性.
- 克雷夫-1cDNA编码了一个21,000达尔顿的蛋白质,大约有50%的氨基酸与ras蛋白质相同.
- 在小鼠,老鼠和的DNA中检测到同源的Krev-1序列,在老鼠器官中无处不在的表达.
结论:
- 克雷夫-1基因在各种组织中起着重要作用.
- 在特定的细胞类型中,Krev-1可能参与细胞生长的负调节.
- 与ras蛋白的结构相似性表明,它可能在与生长控制相关的细胞信号通路中发挥作用.
相关概念视频
02:38The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
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Ras is a superfamily...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
01:20The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
02:38The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...