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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons

Published on: October 31, 2020

用超分辨率成像绘制神经元的突触输入场

Yaron M Sigal¹, Colenso M Speer¹, Hazen P Babcock²

¹Howard Hughes Medical Institute, Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

|October 6, 2015

概括

研究人员使用新型超分辨率平台绘制了小鼠视网膜神经元中的抑制性突触输入. 这揭示了特定的GABAergic输入对于视觉运动检测在开关方向选择性细胞 (DSGCs) 至关重要.

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Super-resolution Imaging of Neuronal Dense-core Vesicles

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科学领域:

神经科学
细胞生物学
视觉系统研究

背景情况:

神经元集成成千上万的突触输入,使突触映射对理解神经计算至关重要.
描述突触输入场,包括身份,强度和位置,对于破译神经元功能至关重要.
视网膜中的开关方向选择性质细胞是处理视觉运动方向的关键.

研究的目的:

为大容量神经成像和突触细分开发一个体积超分辨率重建平台.
在小鼠视网膜中映射开关DSGC的抑制突触输入场.
了解有助于方向选择性的突触的分子特征和受体亚型特异性.

主要方法:

开发一个新的体积超分辨率重建平台.
具有分子身份的神经元和突触的自动细分.
应用该平台对开关DSGC的突触输入进行成像和分析.

主要成果:

该平台能够通过分子信息详细重建大型神经体.
绘制地图显示,开启关闭的DSGC接收特定的GABAergic输入.
这些具有特定受体亚型的GABAergic输入对于产生方向选择性反应至关重要,而无需显著的glycinergic输入用于单突触交叉抑制.

结论:

开发的超高分辨率平台为高分辨率的复杂神经电路提供了独特的功能.
这项研究阐明了DSGC视觉运动检测的具体抑制突触机制.
这些发现突显了GABAergic信号和受体特异性在视觉方向神经计算中的重要性.