由高脂肪饮食引起的红细胞功能障碍:与肥胖相关的动脉样硬化的潜在影响
Dusten Unruh1, Ramprasad Srinivasan1, Tyler Benson1
1From Hematology/Oncology, Department of Internal Medicine, College of Medicine, University of Cincinnati, OH (D.U., R.S., R.K., R.S., V.B., M.P., R.S.F., V.Y.B.); Vascular Biology Center, Georgia Regents University, Augusta, GA (T.B., S.H., T.C., D.J.F., N.L.W.); Cardiovascular Diseases, Department of Internal Medicine, College of Medicine, University of Cincinnati, OH (D.C., N.B., W.T., D.M.); Department of Nutritional Sciences, College of Allied Health Sciences, University of Cincinnati, OH (D.C., A.P.); Department of Pathology, College of Medicine, University of Cincinnati, OH (D.Y.H., W.S.D.); Biomedical Informatics and Developmental Biology / Department of Pediatrics, College of Medicine, University of Cincinnati and Cincinnati Children's Hospital and Medical Center, OH (B.J.A.); Experimental Hematology / Department of Pediatrics, College of Medicine, University of Cincinnati and Cincinnati Children's Hospital and Medical Center, OH (T.K.); Hemoshear LLC, Charlottesville, VA (D.M.); and Department of Emergency Medicine, University of Texas Southwestern Medical Center, Dallas (A.B.).
高脂肪饮食诱导红细胞 (RBC) 功能障碍,增加化学激素结合和炎症相互作用. 这种红细胞功能障碍可能会导致肥胖症的动脉样硬化.
科学领域:
- 心血管科学
- 血液学
- 代谢疾病研究
背景情况:
- 众所周知,高脂肪饮食会促进内皮功能障碍和单细胞激活,导致肥胖症的动脉样硬化.
- 红细胞可以通过达菲抗原受体 (DARC) 作为化学因子储存器.
研究的目的:
- 调查HFD是否会导致红细胞的功能障碍.
- 探索红细胞功能障碍在饮食引起的肥胖和动脉样硬化中的作用.
主要方法:
- 给小鼠提供60%的HFD,持续12周.
- 单细胞化学吸引蛋白-1 (MCP-1) 和与红细胞结合的KC的评估水平.
- 分析了红细胞膜胆固醇,酸外化和脏吸收.
- 在体外和体外评估红细胞-巨细胞相互作用和内皮激活.
主要成果:
- HFD显著增加了依赖DARC的红细胞结合MCP-1和KC.
- 从HFD养小鼠的红细胞显示膜胆固醇和酸外化增加.
- 由HFD诱导的红细胞表现出增强的脏吸收和促进巨对内皮的粘附.
- 这些效应在DARC的小鼠中更为明显.
结论:
- 红细胞功能障碍发生在饮食引起的肥胖早期,类似于内皮功能障碍.
- 功能失调的红细胞可能通过促进炎症相互作用和内皮激活来调解动脉样硬化.
- 这些发现强调了红细胞在与肥胖相关的动脉样硬化病变中的新角色.
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