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Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis
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反向电子转移完成了2,3,5-三四酸替代型核糖酶中的催化循环

Kanchana R Ravichandran, Ellen C Minnihan, Yifeng Wei

  • 1Department of Chemistry and Chemical Biology, Harvard University , 12 Oxford Street, Cambridge, Massachusetts 02138, United States.

Journal of the American Chemical Society
|October 23, 2015
PubMed
概括

这项研究揭示了大肠杆菌核糖核酸酶是如何通过激素途径将核酸二酸盐转化为脱氧核酸的. 引入一种改性氨酸 (F3Y) 允许观察关键的基质中间体,澄清了酶的催化循环.

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