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HIV-1 Nef通过稳定AP-1:Arf1多边形来劫持克拉特林层

Qing-Tao Shen¹, Xuefeng Ren¹, Rui Zhang²

¹Department of Molecular and Cell Biology and California Institute for Quantitative Biosciences, University of California, Berkeley, Berkeley, CA 94720, USA.

Science (New York, N.Y.)

|October 24, 2015

概括

人类免疫缺陷病毒 (HIV) Nef 蛋白和 Arf1 重组了AP-1 克拉特林层. 化EM结构揭示了这些蛋白质如何形成病毒复制和膜流通所必需的六角组件.

科学领域:

细胞生物学
病毒学
结构生物学

背景情况:

像HIV和SIV这样的lentiviruses可以操纵细胞内膜的流动来进行复制.
通过与克拉特林适应蛋白 AP-1 和 AP-2 相互作用,使得 Nef 蛋白能够帮助免疫逃逸.

研究的目的:

阐明HIV-1 Nef和Arf1调节AP-1的结构机制.
确定Arf1和Nef在AP-1三元化,激活和克拉层组装中的作用.

主要方法:

使用冷电子显微镜 (cryo-EM) 来确定Arf1- 和Nef- 结合AP-1的结构.
使用计算模型预测六边形AP-1组件.
进行了体外复制试验以验证克拉组装模型.

主要成果:

艾滋病毒-1 Nef和Arf1诱导三元化和AP-1的激活.
克里奥-EM结构揭示了Arf1分子组织AP-1分离体成一个中心核.
一个六角组装模型被提出,通过直接可视化和克拉复合验证.

结论:

在AP-1的异质激活中,Arf1和Nef具有相互关联的作用.
这些蛋白质对于货物招募和克拉层组装至关重要.
这项研究揭示了克拉特林层内AP-1层的复杂组织.

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