在Arabidopsis中以EIN2为导向的乙烯信号的转化调节
Wenyang Li1, Mengdi Ma1, Ying Feng1
1The State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
Cell
|October 27, 2015
概括
乙烯信号涉及EIN2,一个关键蛋白质,抑制EBF1和EBF2mRNA的翻译. 这一过程利用mRNA 3' UTR和PolyU基因,揭示了一种新的植物激素调节层.
科学领域:
- 植物生物学
- 植物分子生理学
- 生物化学
背景情况:
- 乙烯是一种重要的气态植物激素, 调节生长和发育.
- EIN2是已知的乙烯信号传输信号传感器,通过"切割和穿"模型运行.
- 之前的模型专注于EIN2在转录调节中的作用.
研究的目的:
- 阐明EIN2介导的乙烯信号的替代机制.
- 研究EIN2在目标mRNA的转化调节中的作用.
- 确定这种新途径中涉及的 cis 作用元素和蛋白质因素.
主要方法:
- 对EBF1/2 mRNA 3' UTR进行调控元素的分析.
- 在3' UTR中识别聚尿酸 (PolyU) 基因.
- 共同免疫沉试验用于研究EIN2-mRNA和EIN2-蛋白相互作用.
- 定位研究以追踪细胞质内mRNA和蛋白质的移动,包括到处理体 (P体).
主要成果:
- EIN2直接抑制EBF1和EBF2mRNA的翻译.
- EBF1/ 2 mRNA 的 3' UTR 含有功能性多尿基酸 (PolyU) 基因,可以调节这种抑制.
- 乙烯信号触发了EIN2与这些3' UTR结合.
- 通过与EIN5和PAB等因子的相互作用,EIN2促进EBF1/ 2mRNA对细胞质P体的向.
结论:
- 转化调节是植物中乙烯信号转导的关键组成部分.
- 通过感知细胞信号并传递它们,mRNA 3' UTRs充当"信号传感器".
- 这项研究揭示了EIN2在转化水平上控制基因表达的新机制,扩大了我们对植物激素信号的理解.
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