在Pt (二) 处理后的复合物中,亚酸与酸的功能化:固态结构,光标记和细胞命运
Regina Wirth1, Jonathan D White1, Alan D Moghaddam1
1Department of Chemistry & Biochemistry and Institute of Molecular Biology, University of Oregon , Eugene, Oregon 97403-1253, United States.
Journal of the American Chemical Society
|October 30, 2015
概括
一个新的 (II) 复合物与化组使得有效的光标记和细胞成像. 这种制剂在HeLa细胞中表现出增强的反应性和优异的成像潜力,在细胞核和细胞核中定位.
科学领域:
- 协调化学
- 生物有机化学
- 化学生物学
背景情况:
- 追踪 (II) 复合物对于理解它们与细胞生物分子的相互作用至关重要.
- 使用生物直角反应进行后处理光标签,如铜酸加 (CuAAC) 是一个有前途的策略.
研究的目的:
- 合成和表征一个酸功能化的 ((II) 复合物,cis-[Pt ((2-azidobutyl) amido-1,3-propanediamine) Cl2] (1).
- 将其与之前报告的基功能化同源进行比较.
- 评估它的细胞成像和跟踪潜力.
主要方法:
- 化物功能化Pt(II) 复合物的合成和表征.
- 单晶X射线衍射以确定固态结构.
- 在体外DNA结合研究和使用光标记的点击反应性测试.
- 在使用HeLa细胞和共聚焦光显微镜的研究中.
主要成果:
- 复合物1在固态中呈现出明显的伪链Pt-Pt二聚体排列,与其类同类相比.
- 这两种复合体在体外表现出类似的DNA结合和点击反应性.
- 复合物1在体外表现出较高的点击反应性,与其他化物附加的Pt (II) 复合物相比.
- 在细胞体研究中,发现复合体1具有优越的成像潜力,具有明确的核和核定位.
结论:
- 化物功能化Pt(II) 复合物 (1) 是一种可行的后处理光标记和跟踪剂的工具.
- 复合体1具有有前途的细胞成像能力,突出显示其在各种细胞系中的本地化研究的潜力.
- 这项工作为未来的结细胞目标的隔离和净化铺平了道路.
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