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没有证据表明青春期前小鼠的心肌细胞数量扩大

Kanar Alkass¹, Joni Panula², Mattias Westman³

¹Cell and Molecular Biology, Karolinska Institutet, SE-17177 Stockholm, Sweden; Department of Forensic Medicine, The National Board of Forensic Medicine, SE-17177 Stockholm, Sweden.

|November 7, 2015

概括

心脏细胞的数量很早就建立了,而产后的DNA合成是由多核化和多倍化驱动的,而不是增殖. 这挑战了之前关于心肌细胞生成的发现.

科学领域:

心脏病学
发育生物学
细胞生物学

背景情况:

成人的心脏中心肌细胞生成的程度仍有争议.
一项先前的研究表明,由于小鼠在青春期前的增殖,心肌细胞数量增加了40%.

研究的目的:

研究心肌细胞增殖对产后心脏生长的作用.
重新评估出生后心肌细胞中驱动DNA合成的机制.

主要方法:

使用基于设计的立体学来评估心肌细胞数量和DNA合成.
(15) 用N-胺和氧氨 (BrdU) 分析检测细胞增殖.
在小鼠的产后发育过程中进行了定量评估.

主要成果:

心肌细胞增殖占出生后DNA合成的30%,但在出生后的第11天后心肌细胞数量没有增加.
在青春期前的小鼠中没有观察到繁殖峰值.
心肌细胞多核化 (57%) 和核多重化 (13%) 是产后DNA合成的主要驱动因素.

结论:

大多数心肌细胞是在出生后的一周内形成的.
随后的DNA合成包括多核化和多倍化等非复制性过程.
这些发现与前青少年小鼠显著心肌细胞增殖导致心脏生长的概念相矛盾.

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