在单细胞分化过程中,通过诱导NF-kappa B激活HIV基因表达,从而激活HIV基因表达
G E Griffin1, K Leung, T M Folks
1Howard Hughes Medical Institute, University of Michigan Medical Center, Department of Internal Medicine, Ann Arbor 48109.
Nature
|May 4, 1989
概括
人类免疫缺陷病毒 (HIV) 在单细胞中的复制是由NF-kappa B控制的. 这个转录因子是转录因子.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 分子生物学分子生物学
背景情况:
- 获得性免疫缺陷综合征 (艾滋病) 的潜伏期取决于激活人类免疫缺陷病毒 (HIV) 复制的因素.
- 单细胞细胞是体内HIV生产的潜在储库,但它们对HIV转录的调节仍然不清楚.
研究的目的:
- 为了定义HIV转录在单细胞系中的调节.
- 研究NF-kappa B在单细胞HIV基因表达中的作用.
主要方法:
- 在单细胞系细胞 (U937和U1细胞系) 中研究了HIV基因表达.
- 在单细胞分化过程中研究NF-kappa B结合活性. in vitro.
- 在长期感染的促细胞细胞 (U1) 中评估了HIV-1复制.
主要成果:
- 单细胞中的HIV基因表达受到NF-kappa B的调节,类似于T细胞.
- 在单细胞中,NF-kappa B结合活性在发育过程中受到调节,在分化过程中出现,并在成熟细胞中构成性存在.
- U1细胞的分化与增加的HIV-1复制和NF-kappa B结合活性相关.
结论:
- 在单细胞系中,NF-kappa B在调节HIV转录方面发挥着关键作用.
- NF-kappa B的发育调节有助于单细胞中的HIV激活.
- 了解这一规定可能会为控制艾滋病毒潜伏和复制的策略提供信息.
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