在哺乳动物细胞和酵母细胞中,用于囊泡介导的运输所需的融合蛋白
D W Wilson1, C A Wilcox, G C Flynn
1Department of Biology, Princeton University, New Jersey 08540.
Nature
|June 1, 1989
概括
哺乳动物细胞中囊泡融合所必需的蛋白质被确定为相当于酵母 SEC18. 这一发现揭示了在物种和细胞阶段之间进行囊泡运输的高度保存的机制.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 膀运输对于细胞内分子的移动至关重要,特别是从内质网膜到戈尔吉装置.
- 蛋白质复合体介导运输囊泡与目标器官细胞的融合,如戈尔吉水瓶.
- 了解囊泡融合的分子机械是解读细胞组织和功能的关键.
研究的目的:
- 在一个没有细胞的哺乳动物系统中,识别和描述负责催化囊泡与戈尔吉水瓶融合的蛋白质.
- 为了确定这种哺乳动物融合蛋白是否被保存并且与已知的酵母运输蛋白功能相关.
- 为了阐明囊泡介导的运输机制的进化保存.
主要方法:
- 利用哺乳动物无细胞系统研究囊泡融合中的N-乙烯胺胺敏感蛋白活性.
- 克隆并测序了编码融合蛋白质的基因,从中国仓鼠卵巢细胞中获得.
- 进行了体外测试,以比较哺乳动物蛋白与酵母SEC18基因产品的功能.
主要成果:
- 鉴定了一种对N-乙烯胺胺敏感的蛋白质,该蛋白质催化了输送囊泡与戈尔吉水箱的融合.
- 证明了这种哺乳动物融合蛋白的基因与酵母 (Saccharomyces cerevisiae) 的SEC18基因产物相当.
- 证实了SEC18在囊泡介导的传输中从内质网膜到酵母中的戈尔吉装置的重要作用.
结论:
- 哺乳动物细胞中的囊泡融合催化蛋白与酵母SEC18蛋白在功能上得到保护.
- 囊泡融合的机制在酵母和哺乳动物物种之间高度保留.
- 这种保护延伸到细胞内运输的不同阶段,突出显示了一个基本的生物过程.
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