中性粒细胞激活促进纤维素氧化和血栓形成
Matteo Becatti1, Giacomo Emmi1, Elena Silvestri1
1From Department of Experimental and Clinical Biomedical Sciences "Mario Serio" (M.B., G.B., N.T., C.F.) and Department of Experimental and Clinical Medicine (G.E., E.S., L.C., D.S., R.A., D.P.), University of Florence, Italy; Nephrology Unit, University Hospital of Parma, Italy (A.V.); Interdisciplinary Internal Medicine, Center for Autoimmune Systemic Diseases, Behçet Center and Lupus Clinic, AOU Careggi, Florence, Italy (L.E., D.P.); and Department of Clinical and Experimental Medicine, University Medical School and University Hospital of Parma, Italy (M.G.).
在贝赫特病 (BD) 中,中性粒细胞激活和反应性氧物种 (ROS) 生产会损害纤维素的功能,导致异常的血栓形成和血栓形成的风险增加.
科学领域:
- 免疫学
- 血液学
- 血管生物学
背景情况:
- 贝赫特病 (Behçet disease,简称BD) 是一种全身性血管炎,其特征是免疫炎症过程和高血栓形成风险.
- 氧化还原失衡和中性粒细胞过活化与BD病变和凝血受损有关.
研究的目的:
- 研究免疫细胞激活,特别是中性粒细胞激活和BD中的纤维素修饰之间的联系.
- 确定中性粒细胞激活是否影响纤维素的结构和功能,可能导致BD患者的血栓形成.
主要方法:
- 分析了98名BD患者和70名健康对照者的血液样本.
- 评估纤维素的功能,结构,纤维素对溶解的敏感性,血氧化还原状态和氧化应激标志物.
- 评估中性粒细胞的NADPH氧化酶活性和反应性氧物种 (ROS) 的产生.
主要成果:
- 患有BD的患者表现出血栓催化纤维素形成受损,纤维素对等离子体诱导溶解的敏感性增加.
- 在BD患者中观察到血氧化应激标志物升高和显著的纤维素碳化.
- 中性细胞激活,以增强的NADPH氧化酶活性和ROS产生为标志,与纤维素碳化和凝固能力受损有显著的相关性.
结论:
- 在BD中纤维素结构和功能的改变与中性粒细胞激活和ROS产量的增加有关.
- 中性蛋白NADPH氧化酶被确定为ROS的主要来源,有助于BD的这些变化.
- 这些发现突出了免疫失调与贝赫特病血栓形成的潜在机制.
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