在胰岛素缺乏状态下调节葡萄糖载体信使RNA
W I Sivitz1, S L DeSautel, T Kayano
1Department of Physiology & Biophysics, University of Iowa, Iowa City 52242.
胰岛素通过控制大鼠脂肪组织中的GLUT-4mRNA水平来调节葡萄糖运输. 胰岛素缺乏大大减少GLUT-4mRNA,而胰岛素治疗迅速恢复它.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 生理学 生理学 生理学
背景情况:
- 在哺乳动物中,促进葡萄糖运输涉及一系列相关蛋白质.
- 胰岛素通过将GLUT-4蛋白移动到血膜上来刺激葡萄糖的运输.
- GLUT-4 是脂肪和肌肉组织中主要的胰岛素反应性葡萄糖载体.
研究的目的:
- 在体内研究GLUT-4基因表达通过胰岛素的调节.
- 为了确定胰岛素缺乏和补充对大鼠脂肪组织GLUT-4mRNA水平的影响.
主要方法:
- 测量稳定状态GLUT-4信使RNA (mRNA) 含量,从禁食大鼠和链毒素诱导的糖尿病大鼠的脂肪组织.
- 在胰岛素治疗或再食后评估GLUT-4mRNA水平.
- 与红细胞/HepG2/大鼠大脑类型葡萄糖载体mRNA水平的比较.
主要成果:
- 禁食或胰岛素缺乏导致脂肪组织GLUT-4mRNA水平下降十倍.
- 糖尿病老鼠的胰岛素治疗或重新食的老鼠迅速恢复了GLUT-4mRNA水平.
- 在胰岛素补充后,GLUT-4 mRNA水平恢复到明显高于对照水平.
- 在这些条件下,其他葡萄糖载体mRNA的水平保持不变.
结论:
- 在老鼠脂肪组织中GLUT-4mRNA的体内表达受到胰岛素的调节.
- 胰岛素在控制GLUT-4mRNA的丰富性方面发挥着关键作用,影响葡萄糖运输能力.
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