耳毛细胞的自发活动是由相邻支持细胞中的液体分泌机制触发的
Han Chin Wang1, Chun-Chieh Lin1, Rockie Chong1
1Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 725 North Wolfe Street, WBSB 1001, Baltimore, MD 21205, USA.
Cell
|December 3, 2015
概括
发育中的尾细胞中支持细胞释放的ATP触发了化物通道活动,导致细胞收缩和刺激听觉神经元. 这一过程对于听力系统在听力开始之前的成熟至关重要.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 审计系统开发 审计系统开发
背景情况:
- 自发的神经元活动对于感官系统的发展和连接至关重要.
- 在听觉系统中,由内部支细胞 (ISC) 的ATP驱动的内毛细胞 (IHC) 活动在听觉开始之前成熟了中央通路.
研究的目的:
- 研究内部支持细胞 (ISC) 释放ATP并诱导发育耳神经元的自发活动的机制.
- 阐明TMEM16A活性化化通道在这个过程中的作用.
主要方法:
- 在小鼠模型中利用TMEM16A的遗传删除.
- 测量了IHCs和螺旋质神经元的自发活动.
- 在支持细胞中观察到依赖ATP的细胞收缩.
主要成果:
- 在ISC中,ATP与纯能自身受体结合,打开TMEM16A通道,导致化物和流出.
- 这种外流导致ISC收缩和附近IHCs的短暂脱极化.
- 对TMEM16A的遗传删除显著降低了IHC和螺旋质神经元活动,并防止了支持细胞收缩.
结论:
- 发育中的耳支细胞采用一种类似于液体分泌的途径,涉及TMEM16A,以诱导毛细胞的周期性激发.
- 这种机制对于听觉系统在功能性听觉之前的成熟至关重要.
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