基因必不可少性是与细胞可进化的定量性质
Gaowen Liu1, Mei Yun Jacy Yong2, Marina Yurieva3
1Institute of Medical Biology (IMB), Agency for Science, Technology and Research (A(∗)STAR), Singapore 138648, Singapore; School of Biological Sciences, Nanyang Technological University, Singapore 637551, Singapore.
Cell
|December 3, 2015
概括
酵母中的大多数"基本"基因可以通过适应性进化克服,从而揭示了基因可进化的梯度. 这表明重新定义基因必不可少性以包括更好的药物标选择的可进化性.
科学领域:
- 遗传学
- 进化生物学
- 系统生物学
背景情况:
- 基因关键性通常由基因删除后的细胞活力定义.
- 这种定义忽视了细胞因基因变化而适应和进化的能力.
- 了解适应性进化对于全面了解基因功能至关重要.
研究的目的:
- 在Saccharomyces cerevisiae中定量评估基本基因的可变性.
- 研究基因缺失所导致的适应机制.
- 提出一个重新定义的基因必不可少性概念,
主要方法:
- 进行了严格的基因选,以评估酵母中1000个基本基因被删除后的存活率.
- 分析了进化突变菌株的适应性突变和性变化.
- 量化了基因基本性和可进化的全基因组梯度.
主要成果:
- 大约9%的基本基因缺失通过适应性进化得到克服.
- 确定了全基因组梯度,表明基因可演化的不同水平.
- 在核突变体中观察到流行性形变化,包括适应性形变化.
结论:
- 基因必不可少性不是绝对的,可以受到适应性进化的影响.
- 对基因必不可少性的量化重新定义应该包括可活性和可进化性.
- 这种修订的理解可以帮助选择具有抗药性出现风险较低的治疗点.
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