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表皮IL-18平衡控制大肠炎中的屏障功能

Roni Nowarski1, Ruaidhrí Jackson1, Nicola Gagliani1

  • 1Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA.

Cell
|December 7, 2015
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概括
此摘要是机器生成的。

通过破坏肠道屏障来驱动性结肠炎. 阻断肠上皮细胞中的IL-18信号,可以防止结肠炎并保护杯状细胞.

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科学领域:

  • 胃肠病学
  • 免疫学
  • 细胞生物学

背景情况:

  • 肠道粘膜屏障对于控制微生物群至关重要, 它依赖于产生粘液的杯状细胞.
  • 炎症性肠道疾病,特别是性结肠炎的特征是杯状细胞功能受损和粘液层分解.

研究的目的:

  • 调查介素-18 (IL-18) 在结肠炎期间肠道屏障完整性的病理性破坏中的作用.
  • 阐明肠上皮细胞中IL-18信号影响杯细胞成熟和结肠炎严重性的机制.

主要方法:

  • 使用大肠炎的小鼠模型.
  • 在肠道上皮细胞中产生特定缺失的Il18,Il18r1 (IL-18受体1) 和Il18bp (IL-18结合蛋白) 的转基因小鼠.
  • 通过组织学和分子分析评估大肠炎的严重程度,粘膜损伤和杯状细胞成熟.

主要成果:

  • 在肠道上皮细胞中删除Il18或Il18r1可显著保护大肠炎和粘膜损伤.
  • 删除Il18bp导致严重的结肠炎和成熟杯细胞的丧失.
  • 在肠道上皮细胞中同时删除Il18bp和Il18r1挽救了结肠炎和杯状细胞损失,证实了上皮细胞中的IL-18信号作为关键调节器.
  • 通过改变控制杯状细胞发育的转录程序,发现IL-18可以抑制杯状细胞的成熟.

结论:

  • 肠上皮细胞中的IL-18信号是大肠炎病理和肠壁功能障碍的关键驱动因素.
  • 向肠上皮细胞中的IL-18信号是一种潜在的治疗策略.
  • 了解IL-18对小杯细胞发育的调节是解决性结肠炎中小杯细胞功能障碍的关键.