通过模板辅助氧化聚合物进行siRNA输送的可减少裂变纳米囊
P K Hashim1, Kou Okuro1, Shigekazu Sasaki2
1Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Journal of the American Chemical Society
|December 10, 2015
概括
研究人员开发了富含guanidinium的新型聚合物来封装小干扰RNA (siRNA). 这些聚合物形成纳米囊,将siRNA输送到细胞中,并在细胞质中释放,以有效地抑制基因表达.
科学领域:
- 生物材料科学
- 聚合物化学
- 基因传递
背景情况:
- 在基因沉默疗法中,siRNA传递至关重要.
- 开发高效和安全的siRNA载体仍然是一个挑战.
- 基可以增强细胞吸收和内体逃生.
研究的目的:
- 用多个关尼单元合成水溶性滴醇单体.
- 为siRNA包装开发一个模板辅助氧化聚合方法.
- 评估 siRNA 装载纳米的细胞吸收和基因沉默功效.
主要方法:
- 用瓜尼尼单元合成电二醇单体.
- 使用siRNA作为模板进行模板辅助氧化聚合.
- 纳米囊大小和siRNA负载的表征.
- 细胞吸收和基因表达抑制的体外研究.
主要成果:
- 均大小 (7 ± 2 nm) 的纳米囊封装siRNA成功形成.
- 这些纳米囊在活细胞中表现出高效的细胞吸收.
- 细胞内脱聚合释放的siRNA在还原性细胞环境中.
- 观察到显著的基因表达抑制.
结论:
- 新型富含guanidinium的聚合物可以有效地包装和传递siRNA.
- 开发的纳米囊是基因沉默应用的有效载体.
- 这种方法为治疗siRNA提供了一个有前途的策略.
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