相关实验视频

Updated: Mar 28, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

与RNA聚合酶II相关的因子1调节了暂停的RNA聚合酶II的释放和酸化

Ming Yu¹, Wenjing Yang², Ting Ni³

¹Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10065, USA.

Science (New York, N.Y.)

|December 15, 2015

概括

对于基因调节至关重要的Pol II暂停释放涉及新的因素. 积极的转录延长因子b (P-TEFb) 招募PAF1复合体 (PAF1C),然后招募CDK12,显示出一个合作机制.

科学领域:

分子生物学
基因调控
生物化学

背景情况:

在甲基动物中,转录调节涉及促进体近位暂停RNA聚合酶II (Pol II) 的释放.
传统上认为循环酶依赖性酶9 (CDK9) 活性对这种释放至关重要,化关键因素.
了解控制 Pol II 暂停释放的精确分子机制对于破译基因表达控制至关重要.

研究的目的:

研究Pol II相关因子1 (PAF1) 在暂停的Pol II释放中的作用.
在转录暂停中阐明P-TEFb,PAF1复合体 (PAF1C) 和CDK12之间的调节相互作用.
在早期的转录延长中发现新的合作机制.

主要方法:

这项研究可能涉及生物化学测试,基因操纵和分子生物学技术,以评估蛋白质相互作用和基因表达.
研究了PAF1C和CDK12对基因的招募动态.
研究了PAF1和P-TEFb对Pol IICTD酸化的影响.

主要成果:

被确定为暂停的Pol II释放的关键调节者.
阳性转录延长因子b (P-TEFb) 直接介导PAF1复合体 (PAF1C) 到基因的初始招募.
发现CDK12对基因的注入取决于PAF1C.

结论:

发现了一种涉及P-TEFb,PAF1C和CDK12在暂停的Pol II释放中的新合作机制.
这种合作性对于高效的Pol IICTD酸化和早期的转录延长至关重要.
这些发现扩大了我们对复杂的基因转录调节的理解.

更多相关视频

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

High-throughput Purification of Affinity-tagged Recombinant Proteins

High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

相关概念视频

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

RNA Polymerase II Accessory Proteins

RNA Polymerase II Accessory Proteins

Eukaryotic RNA Polymerases

Eukaryotic RNA Polymerases

RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...

Eukaryotic RNA Polymerases

Eukaryotic RNA Polymerases

Transcription Elongation Factors

Transcription Elongation Factors

Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...

Transcription Elongation Factors

Transcription Elongation Factors