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细胞对细胞的转录变量:在噪声中看到信号

Kevin A Janes¹

¹Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA.

|December 22, 2015

概括

哺乳动物细胞中的基因表达令人惊地可预测. 细胞状态和环境缓冲转录噪声,使mRNA水平不像以前想象的那样随机.

科学领域:

分子生物学
细胞生物学
遗传学

背景情况:

基因表达的随机性是细胞功能的一个基本方面.
了解这种噪声的来源和调节对于理解细胞间的变化至关重要.
核在哺乳动物细胞中缓冲转录噪声中的作用仍然是一个活跃的研究领域.

研究的目的:

研究哺乳动物细胞内基因表达的随机性程度.
通过细胞特征来预测mRNA水平的单细胞变异性.
阐明基因表达噪声中的机制,特别是核的作用.

主要方法:

单细胞mRNA水平的定量分析.
细胞表型状态的测量.
影响基因表达的微环境因素的评估.

主要成果:

细胞质mRNA水平的单细胞变异性高度可预测.
细胞表型状态和微环境是mRNA变异性的关键预测因素.
核作为一个缓冲器,减轻转录爆发引起的噪音.

结论:

哺乳动物细胞中的基因表达率低于预期.
mRNA水平的可预测性表明强大的调节机制正在发挥作用.
通过缓冲转录噪声,真核在稳定基因表达方面发挥着重要作用.

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