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来自Arabidopsis thaliana的电压门式双孔通道TPC1的结构

Jiangtao Guo¹, Weizhong Zeng^1,2, Qingfeng Chen^1,2

¹Department of Physiology, University of Texas Southwestern Medical Center, Dallas, Texas 75390-9040, USA.

|December 23, 2015

概括

植物真空双孔通道 (TPC) 的晶体结构揭示了电压和如何激活它. 这为电压门通道机制提供了新的见解.

科学领域:

分子生物学分子生物学
结构生物学结构生物学
植物生理学植物生理学

背景情况:

双孔通道 (TPCs) 是动物和植物中发现的重要器官质离子通道.
TPC 具有独特的结构,每个子单元有两个类似 Shaker 的六个跨膜 (6-TM) 域.
了解TPC功能对于细胞离子恒温和信号传递至关重要.

研究的目的:

为了确定Arabidopsis thaliana (AtTPC1) 的真空双孔通道的晶体结构.
阐明由电压和激活AtTPC1背后的分子机制.
为TPC的电压调节机制提供结构性见解.

主要方法:

使用X射线结晶学来获得AtTPC1.1的结构.
生物化学和生物物理技术被用来研究道激活.
结构分析的重点是和电压与TPC领域的相互作用.

主要成果:

确定了作为同位体的AtTPC1的晶体结构.
在TPC1激活是一个双重过程,涉及电压和细胞质Ca2+).
2+) 结合会影响第一个6-TM域,而膜电位会影响第二个电压感应域.

结论:

这项研究揭示了电压和Ca2+在AtTPC1关门中的不同作用.
光线Ca2+) 或Ba2+) 可以调节电压激活,稳定静止状态.
2+) 结合结构为电压传感器静止状态和一般电压门通道机制提供了前所未有的洞察力.

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