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聚A结合蛋白对于聚A缩短和60S核糖体子单元依赖的翻译启动是必需的

A B Sachs¹, R W Davis

¹Department of Biochemistry, Stanford University Medical Center, California 94305.

|September 8, 1989

概括

聚乙烯结合蛋白 (PAB) 对于酵母中的翻译启动至关重要. 抑制器突变显示,60S核糖体子单元调解了有效的mRNA翻译的PAB要求.

科学领域:

分子生物学分子生物学
酵母遗传学酵母遗传学
蛋白质合成蛋白质合成

背景情况:

基本的多A结合蛋白 (PAB) 在mRNA调节中起着至关重要的作用.
在S. cerevisiae中,PAB的枯竭抑制了翻译的启动,并缩短了多尾.

研究的目的:

调查PAB在翻译启动时所需的机制.
确定可以抑制PAB功能丧失的遗传因素.

主要方法:

利用对温度敏感的突变 (pab1-F364L) 和促进体无活化来耗尽S. cerevisiae中的PAB.
进行逆转分析以隔离对寒冷敏感的抑制器突变 (spb1-spb7).
分析抑制基因突变对翻译启动,多基因A尾巴长度和核糖体子单元组成的影响.

主要成果:

鉴定出7种外源性冷敏突变 (spb1-spb7),可以抑制PAB的删除.
这些突变使得在没有PAB的情况下可以启动翻译,而不会改变多个A的尾巴长度.
发现抑制器突变会影响60S核糖体子单元的数量,SPB2编码核糖体蛋白L46.

结论:

60S核糖体子单元参与调解在翻译启动过程中对PAB的要求.
这种相互作用确保了完整的多元A) +mRNA在体内有效的翻译.
了解这种途径可以提供对mRNA质量控制和翻译调节的见解.

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