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Updated: Jul 19, 2026
12:19
Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
视网母细胞瘤蛋白在细胞周期的特定阶段被酸化
K Buchkovich1, L A Duffy, E Harlow
1Cold Spring Harbor Laboratory, New York 11724.
Cell
|September 22, 1989
概括
视网母细胞瘤瘤抑制蛋白 (p105-RB) 存在于分裂细胞中. 它的酸化状态在细胞周期期间发生变化,这表明它在细胞分裂中起着调节作用.
科学领域:
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
- 癌症研究 癌症研究
背景情况:
- 视网母细胞瘤瘤抑制基因产物p105-RB是一种核蛋白.
- 假设它抑制细胞增殖,但是在活跃分裂的细胞中发现的.
研究的目的:
- 为了研究细胞周期期间p105-RB的变化.
- 了解p105-RB活动是如何由细胞周期进展调节的.
主要方法:
- 同步细胞群的生成.
- 在整个细胞周期中监测p105-RB表达和酸化.
主要成果:
- 在细胞周期中,p105-RB是连续合成的.
- 在G0和G1阶段检测到非酸化p105-RB.
- 多种化形式的p105-RB出现在S和G2/M阶段.
结论:
- p105-RB酸化是细胞周期依赖的.
- 对p105-RB的相位特异酸化可能调节其在细胞分裂中的功能.
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01:04What is the Cell Cycle?
The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
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The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...
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The cell cycle refers to the sequence of events occurring throughout a typical cell’s life. In eukaryotic cells, the somatic cell cycle has two stages: the interphase and the mitotic phase. During interphase, the cell grows, performs its basic metabolic functions, copies its DNA, and prepares for mitotic cell division. Then, during mitosis and cytokinesis, the cell divides its nuclear and cytoplasmic materials, respectively. This generates two daughter cells that are identical to the original...