编码酵母TATA结合因子TFIID的基因SPT15是体内正常转录启动所需的
D M Eisenmann1, C Dollard, F Winston
1Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Cell
|September 22, 1989
概括
发现编码TATA结合因子TFIID的SPT15基因的突变会改变基因转录. 这种必不可少的转录因子对于酵母的生长,交配和分泌至关重要,影响了许多基因.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 酵母生物学的酵母生物学
背景情况:
- 研究Saccharomyces cerevisiae中的基因调节机制.
- 了解转录因子在控制基因表达中的作用.
- 此前,SPT15基因已在改变基因转录的抑制器屏幕中被确定.
研究的目的:
- 为了识别和描述SPT15基因.
- 为了确定SPT15基因产品在转录启动中的功能.
- 评估SPT15突变的基本性和类效应.
主要方法:
- 孤立SPT15突变作为插入突变的抑制剂.
- 对SPT15基因的分子克隆和遗传分析.
- 对spt15突变菌株的表型分析,包括生长,交配和分泌试验.
主要成果:
- SPT15基因编码的是TATA结合因子TFIID.
- 在TFIID的变化 (通过spt15突变) 影响体内转录启动.
- TFIID对于酵母生长至关重要;spt15突变体表现出缓慢的生长和交配和化的缺陷.
结论:
- TFIID是Saccharomyces cerevisiae活力的重要转录因子.
- TFIID在调节体内转录启动方面发挥着至关重要的作用.
- SPT15突变揭示了TFIID在基底转录之外的类作用.
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